A cell-free approach to accelerate the study of protein-protein interactions in vitro.
نویسندگان
چکیده
Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
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عنوان ژورنال:
- Interface focus
دوره 3 5 شماره
صفحات -
تاریخ انتشار 2013